Cryopreservation and Transplantation of Ovarian Tissue
September 12, 2018
CHUM – Pavillon R
900, rue St-Denis
Montréal (Québec) H2X 0A9
Ovarian tissue cryopreservation and transplantation for fertility purposes has been performed for more than 20 years. Its success rate is around 30% after autotransplantation of frozen-thawed ovarian cortex, with more than 100 live births reported to date. This workshop aims to provide a high-level overview on cryopreservation and transplantation of human ovarian tissue. We will share our experience on how to start and organize an ovarian tissue cryobank, prepare, freeze and thaw biopsies and perform ovarian tissue transplantation. To ensure the proper training of all participants, we offer hands-on practice on cryopreservation. Additionally, the participants can learn new alternatives procedures, such as ovarian tissue vitrification and in vitro culture/xenografting.
- Identify fertility preservation options for female cancer patients;
- Select the cryopreservation and transplantation of ovarian tissue (CTOT) strategy for cancer patients and patients with benign diseases;
- Know crucial steps to set up a CTOT program; and
- Cryopreserve and transplant ovarian tissue.
Who should Attend: Biologists, embryologists, gynecologists, surgeons and scientists.
Registration: Only 15 spots available! Priority will be given to CFAS Members
Registration Fee: $250 for Members, $450 for Non-Members
About the Presenter:
Christiani Amorim, PhD is a leading authority on ovarian tissue and its transplantation for women.
She has taught theoretical and practical courses in cryopreservation and transplantation of ovarian tissue in Europe, China, Brazil, and Mexcio. Christiani has a PhD in veterinary medicine and has been working with human preantral follicles with a major emphasis on human follicle isolation, cryopreservation, in-vitro culture and xenotransplantation since 2007.
|09:00 – 09:30||Overview on fertility preservation strategies|
|09:30 – 10:10||Overview on ovarian tissue cryopreservation|
|10:10 – 10:40||Freezing and thawing of human ovarian tissue|
|10:40 – 11:10||Coffee break|
|11:10 – 11:30||Ovarian tissue cryobanking according to European directives|
|11:30 – 12:00||Fertility preservation in prepubertal girls|
|12:00 – 13:30||Lunch|
|13:30 – 15:00||Preparation of the ovarian biopsy before freezing (hands on practice with cow ovaries)|
|15:00 – 15:30||Coffee break|
|15:30 – 17:00||Freezing and thawing of ovarian tissue (hands on practice with cow ovaries)|