Cryopreservation and Transplantation of Ovarian Tissue

September 12, 2018

CHUM – Pavillon R
900, rue St-Denis
Montréal (Québec) H2X 0A9

Ovarian tissue cryopreservation and transplantation for fertility purposes has been performed for more than 20 years. Its success rate is around 30% after autotransplantation of frozen-thawed ovarian cortex, with more than 100 live births reported to date. This workshop aims to provide a high-level overview on cryopreservation and transplantation of human ovarian tissue. We will share our experience on how to start and organize an ovarian tissue cryobank, prepare, freeze and thaw biopsies and perform ovarian tissue transplantation. To ensure the proper training of all participants, we offer hands-on practice on cryopreservation. Additionally, the participants can learn new alternatives procedures, such as ovarian tissue vitrification and in vitro culture/xenografting.

Learning Objectives: 

  1. Identify fertility preservation options for female cancer patients;
  2. Select the cryopreservation and transplantation of ovarian tissue (CTOT) strategy for cancer patients and patients with benign diseases;
  3. Know crucial steps to set up a CTOT program; and
  4. Cryopreserve and transplant ovarian tissue.

Who should Attend: Biologists, embryologists, gynecologists, surgeons and scientists.

Registration: Only 15 spots available! Priority will be given to CFAS Members

Registration Fee: $250 for Members, $450 for Non-Members



About the Presenter:

Christiani Amorim, PhD is a leading authority on ovarian tissue and its transplantation for women.
She has taught theoretical and practical courses in cryopreservation and transplantation of ovarian tissue in Europe, China, Brazil, and Mexcio. Christiani has a PhD in veterinary medicine and has been working with human preantral follicles with a major emphasis on human follicle isolation, cryopreservation, in-vitro culture and xenotransplantation since 2007.

Time Program
09:00 – 09:30 Overview on fertility preservation strategies
09:30 – 10:10 Overview on ovarian tissue cryopreservation
10:10 – 10:40 Freezing and thawing of human ovarian tissue
10:40 – 11:10 Coffee break
11:10 – 11:30 Ovarian tissue cryobanking according to European directives
11:30 – 12:00 Fertility preservation in prepubertal girls
12:00 – 13:30 Lunch
13:30 – 15:00 Preparation of the ovarian biopsy before freezing (hands on practice with cow ovaries)
15:00 – 15:30 Coffee break
15:30 – 17:00 Freezing and thawing of ovarian tissue (hands on practice with cow ovaries)